Requirement for PLK1 kinase activity in the maintenance of a robust spindle assembly checkpoint
نویسندگان
چکیده
منابع مشابه
A requirement for MAP kinase in the assembly and maintenance of the mitotic spindle
Circumstantial evidence has suggested the possibility of microtubule-associated protein (MAP) kinase's involvement in spindle regulation. To test this directly, we asked whether MAP kinase was required for spindle assembly in Xenopus egg extracts. Either the inhibition or the depletion of endogenous p42 MAP kinase resulted in defective spindle structures resembling asters or half-spindles. Like...
متن کاملThe spindle assembly checkpoint
Italian in 1954, so Imanishi turned to the exploration of the unexplored wide range of Karakorum Himalayas, traversing the three major glaciers in 1955. Then, in 1958, Imanishi and Itani went to Africa for the first time. In the same year, Nishibori led the all-Japanese team which succeeded in the first over-wintering stay in Antarctica. Kuwabara led the Himalayan expedition of AACK to the firs...
متن کاملThe Spindle Assembly Checkpoint
During mitosis and meiosis, the spindle assembly checkpoint acts to maintain genome stability by delaying cell division until accurate chromosome segregation can be guaranteed. Accuracy requires that chromosomes become correctly attached to the microtubule spindle apparatus via their kinetochores. When not correctly attached to the spindle, kinetochores activate the spindle assembly checkpoint ...
متن کاملLoss of the Greatwall Kinase Weakens the Spindle Assembly Checkpoint
The Greatwall kinase/Mastl is an essential gene that indirectly inhibits the phosphatase activity toward mitotic Cdk1 substrates. Here we show that although Mastl knockout (MastlNULL) MEFs enter mitosis, they progress through mitosis without completing cytokinesis despite the presence of misaligned chromosomes, which causes chromosome segregation defects. Furthermore, we uncover the requirement...
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Polyploidization in megakaryocytes is achieved by endomitosis, a specialized cell cycle in which DNA replication is followed by aberrant mitosis. Typical mitotic regulators such as Aurora kinases or Cdk1 are dispensable for megakaryocyte maturation, and inhibition of mitotic kinases may in fact promote megakaryocyte maturation. However, we show here that Polo-like kinase 1 (Plk1) is required fo...
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ژورنال
عنوان ژورنال: Biology Open
سال: 2015
ISSN: 2046-6390
DOI: 10.1242/bio.014969